本发明涉及一种基于核酸适体自催化作用的癌胚抗原检测试剂盒及其制备方法。癌胚抗原检测试剂盒，包括：第一适体由核苷酸序列如SEQ ID NO.1所示的核苷酸片段的3’端通过羟基结合一个生物素构成；第二适体由核苷酸序列如SEQ ID NO.2所示的核苷酸片段构成；链霉亲和素包被的磁珠；KCl溶液、氯化血红素溶液、反应缓冲液、显色液、终止反应液。本发明所述试剂盒对癌胚抗原进行检测，较现有检测方法，无需动物免疫抗体和酶标记过程，核酸适体为化学方法合成，稳定性和环境耐受性更好，且试剂保存更为方便，定量检测仅需要一台可见分光光度计即可。
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