本发明公开了一种抗CD137单克隆抗体的生物活性测定方法，其包括以下步骤：将CCRF CEM NF‑KB细胞悬液行离心处理，弃上清液，完全培养基重悬细胞，并加入PMA和Ionomycin的混合液刺激细胞，然后离心处理，弃上清液；将交联抗体和CD137抗体加入完全培养基中制得交联组抗体溶液，梯度稀释得抗体溶液；将CD137抗体加入完全培养基中制得非交联组抗体溶液，梯度稀释得抗体溶液；取抗体溶液分别与所得细胞悬液混合均匀，静置培养，然后加入荧光素酶底物，孵育，再检测化学发光的表达。本发明的有益效果为：本发明所述方法通过激活CD137信号激活转录因子NFKB启动荧光素酶的表达，其表达量与抗体的生物活性呈正相关，专属性强、耐用性高，对于单克隆抗体的质量控制和临床应用具有重要意义。
[8]Trying to access array offset on value of type null[/websites/wwwroot/www.china-mcc.com/runtime/temp/d969c9ac11613d3901d9652da6568a9b.php:214]
[8]Trying to access array offset on value of type null[/websites/wwwroot/www.china-mcc.com/runtime/temp/d969c9ac11613d3901d9652da6568a9b.php:218]
[8]Trying to access array offset on value of type null[/websites/wwwroot/www.china-mcc.com/runtime/temp/d969c9ac11613d3901d9652da6568a9b.php:237]
[8]Array to string conversion[/websites/wwwroot/www.china-mcc.com/thinkphp/library/think/Url.php:128]
[8]Array to string conversion[/websites/wwwroot/www.china-mcc.com/thinkphp/library/think/Url.php:147]
[8]Array to string conversion[/websites/wwwroot/www.china-mcc.com/thinkphp/library/think/Url.php:147]
[8]Array to string conversion[/websites/wwwroot/www.china-mcc.com/thinkphp/library/think/Url.php:128]
[8]Array to string conversion[/websites/wwwroot/www.china-mcc.com/thinkphp/library/think/Url.php:147]
[8]Array to string conversion[/websites/wwwroot/www.china-mcc.com/thinkphp/library/think/Url.php:147]
[8]Array to string conversion[/websites/wwwroot/www.china-mcc.com/thinkphp/library/think/Url.php:128]
[ DB ] CONNECT:[ UseTime:0.001162s ] mysql:host=pc-m5epcw1px949b110u.rwlb.rds.aliyuncs.com;port=3306;dbname=yousedb;charset=utf8
[ SQL ] SHOW COLUMNS FROM `tb_para` [ RunTime:0.001904s ]
[ SQL ] SELECT * FROM `tb_para` WHERE `id` = 1 LIMIT 1 [ RunTime:0.001195s ]
[ SQL ] SHOW COLUMNS FROM `tb_tech` [ RunTime:0.001066s ]
[ SQL ] UPDATE `tb_tech` SET `hit`=`hit`+1 WHERE `id` = 874480 [ RunTime:0.001216s ]
[ SQL ] SELECT * FROM `tb_tech` WHERE `id` = 874480 LIMIT 1 [ RunTime:0.000866s ]
[ SQL ] SHOW COLUMNS FROM `tb_admin` [ RunTime:0.001187s ]
[ SQL ] SELECT `realname` FROM `tb_admin` WHERE `id` = 1 LIMIT 1 [ RunTime:0.000711s ]
[ SQL ] SHOW COLUMNS FROM `tb_category` [ RunTime:0.000998s ]
[ SQL ] SELECT * FROM `tb_category` WHERE `id` = 147 LIMIT 1 [ RunTime:0.000675s ]
[ SQL ] SELECT * FROM `tb_category` WHERE `id` = 30 LIMIT 1 [ RunTime:0.000789s ]
[ SQL ] SHOW COLUMNS FROM `tb_anchor` [ RunTime:0.000948s ]
[ SQL ] SELECT * FROM `tb_anchor` ORDER BY `id` ASC [ RunTime:0.002418s ]
[ SQL ] SELECT `id`,`title` FROM `tb_tech` WHERE `id` > 874480 AND `check_status` = 1 AND `category_id` = 147 ORDER BY `submit_date` ASC LIMIT 1 [ RunTime:0.005420s ]
[ SQL ] SELECT `id`,`title` FROM `tb_tech` WHERE `id` < 874480 AND `check_status` = 1 AND `category_id` = 147 ORDER BY `submit_date` DESC LIMIT 1 [ RunTime:0.080371s ]
[ SQL ] select id,title,tag,submit_date from tb_tech where check_status=1 and id<>874480 order by submit_date desc limit 10 [ RunTime:0.016008s ]
[ SQL ] SHOW COLUMNS FROM `tb_mcc` [ RunTime:0.001313s ]
[ SQL ] SELECT * FROM `tb_mcc` WHERE `classify` = 1 [ RunTime:0.000799s ]
[ SQL ] SELECT * FROM `tb_mcc` WHERE `classify` = 2 [ RunTime:0.000799s ]
[ DB ] CONNECT:[ UseTime:0.001309s ] mysql:host=mccbfzy.mysql.polardb.rds.aliyuncs.com;port=3306;dbname=ysscdb;charset=utf8
[ SQL ] SHOW COLUMNS FROM `tb_industry_report` [ RunTime:0.001103s ]
[ SQL ] SELECT `id`,`cate_aid`,`cate_bid`,`title`,`pic`,`tag`,`price`,`hit`,`mode` FROM `tb_industry_report` [ RunTime:0.001207s ]
[ SQL ] SELECT * FROM `tb_category` WHERE `pid` = 36 ORDER BY `sortid` ASC [ RunTime:0.000864s ]
[ SQL ] SELECT * FROM `tb_category` WHERE `pid` = 37 ORDER BY `sortid` ASC [ RunTime:0.000882s ]
[ SQL ] SELECT * FROM `tb_category` WHERE `pid` = 38 ORDER BY `sortid` ASC [ RunTime:0.000834s ]
[ SQL ] SELECT * FROM `tb_category` WHERE `pid` = 39 ORDER BY `sortid` ASC [ RunTime:0.000864s ]
[ SQL ] SELECT * FROM `tb_category` WHERE `pid` = 40 ORDER BY `sortid` ASC [ RunTime:0.000811s ]
[ SQL ] SELECT * FROM `tb_category` WHERE `pid` = 41 ORDER BY `sortid` ASC [ RunTime:0.000838s ]
[ SQL ] SHOW COLUMNS FROM `tb_advert` [ RunTime:0.001117s ]
[ SQL ] SELECT * FROM `tb_advert` WHERE `id` = 62 LIMIT 1 [ RunTime:0.000792s ]
[ SQL ] SELECT * FROM `tb_advert` WHERE `id` = 63 LIMIT 1 [ RunTime:0.000843s ]
[ SQL ] SELECT * FROM `tb_advert` WHERE `id` = 64 LIMIT 1 [ RunTime:0.000694s ]
[ SQL ] SELECT * FROM `tb_advert` WHERE `id` = 65 LIMIT 1 [ RunTime:0.000771s ]
849
编辑：管理员
来源：北方有色网
咨询细节
2026年08月06日 ~ 08日 
