本发明公开一种人巨细胞病毒UL49基因抗原肽、及其抗体与应用,该抗原肽具有如SEQ ID NO.1所示的氨基酸序列,其核苷酸序列如SEQ ID NO.2所示。本发明的抗原肽为12短肽,易于化学合成,且产物纯度高。利用本发明的抗原肽制备的抗体,其效价可达1∶8000以上,对UL49原核表达蛋白、真核表达蛋白以及HCMV颗粒都具有高特异性,可用于制备体外诊断或治疗人巨细胞病毒的药物。本发明的抗原肽制备的抗体是HCMV UL49生物学功能研究的基础要素,利用该抗体,可以对HCMV感染进行定性或定量检测。
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[ DB ] CONNECT:[ UseTime:0.001521s ] mysql:host=pc-m5epcw1px949b110u.rwlb.rds.aliyuncs.com;port=3306;dbname=yousedb;charset=utf8
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[ SQL ] SELECT * FROM `tb_para` WHERE `id` = 1 LIMIT 1 [ RunTime:0.001282s ]
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[ SQL ] SELECT * FROM `tb_tech` WHERE `id` = 885335 LIMIT 1 [ RunTime:0.001077s ]
[ SQL ] SHOW COLUMNS FROM `tb_admin` [ RunTime:0.001107s ]
[ SQL ] SELECT `realname` FROM `tb_admin` WHERE `id` = 1 LIMIT 1 [ RunTime:0.000809s ]
[ SQL ] SHOW COLUMNS FROM `tb_category` [ RunTime:0.001041s ]
[ SQL ] SELECT * FROM `tb_category` WHERE `id` = 147 LIMIT 1 [ RunTime:0.000780s ]
[ SQL ] SELECT * FROM `tb_category` WHERE `id` = 30 LIMIT 1 [ RunTime:0.000743s ]
[ SQL ] SHOW COLUMNS FROM `tb_anchor` [ RunTime:0.000955s ]
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[ SQL ] SELECT `id`,`title` FROM `tb_tech` WHERE `id` > 885335 AND `check_status` = 1 AND `category_id` = 147 ORDER BY `submit_date` ASC LIMIT 1 [ RunTime:0.007471s ]
[ SQL ] SELECT `id`,`title` FROM `tb_tech` WHERE `id` < 885335 AND `check_status` = 1 AND `category_id` = 147 ORDER BY `submit_date` DESC LIMIT 1 [ RunTime:0.043971s ]
[ SQL ] select id,title,tag,submit_date from tb_tech where check_status=1 and id<>885335 order by submit_date desc limit 10 [ RunTime:0.004521s ]
[ SQL ] SHOW COLUMNS FROM `tb_mcc` [ RunTime:0.001208s ]
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[ DB ] CONNECT:[ UseTime:0.001139s ] mysql:host=mccbfzy.mysql.polardb.rds.aliyuncs.com;port=3306;dbname=ysscdb;charset=utf8
[ SQL ] SHOW COLUMNS FROM `tb_industry_report` [ RunTime:0.000919s ]
[ SQL ] SELECT `id`,`cate_aid`,`cate_bid`,`title`,`pic`,`tag`,`price`,`hit`,`mode` FROM `tb_industry_report` [ RunTime:0.001027s ]
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[ SQL ] SELECT * FROM `tb_advert` WHERE `id` = 65 LIMIT 1 [ RunTime:0.000836s ]
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