本发明公开了一种化学合成的HBV 1.2x基因组、表达系统及构建方法，所述HBV 1.2x基因组的DNA序列为SEQ ID NO：1所示的第一序列。所述第一序列由SEQ ID NO：2所示的第二序列改造而成，所述改造序列(对应第二序列的1501-3215，1-2150)，其中1501-3215片段的第1909个核苷酸由T突变为C、第1912个核苷酸由C突变为T、第2248个核苷酸由T突变为C，在改造序列的两端均加入序列GATATC得到所述第一序列。根据本发明HBV1.2x基因组构建重组质粒pcDNA3.1(+)/HBV，然后转入肝肿瘤细胞系HepG2细胞，培养上清中检测到HBV特异性抗原HBe Ag和HBs Ag，从而能够实现HBV特异性抗原的表达。
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[8]Array to string conversion[/websites/wwwroot/www.china-mcc.com/thinkphp/library/think/Url.php:147]
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[8]Array to string conversion[/websites/wwwroot/www.china-mcc.com/thinkphp/library/think/Url.php:147]
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[ DB ] CONNECT:[ UseTime:0.001199s ] mysql:host=pc-m5epcw1px949b110u.rwlb.rds.aliyuncs.com;port=3306;dbname=yousedb;charset=utf8
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[ SQL ] SELECT * FROM `tb_para` WHERE `id` = 1 LIMIT 1 [ RunTime:0.001155s ]
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[ SQL ] SHOW COLUMNS FROM `tb_category` [ RunTime:0.000927s ]
[ SQL ] SELECT * FROM `tb_category` WHERE `id` = 147 LIMIT 1 [ RunTime:0.000774s ]
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[ SQL ] SHOW COLUMNS FROM `tb_anchor` [ RunTime:0.000895s ]
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[ SQL ] SELECT `id`,`title` FROM `tb_tech` WHERE `id` > 855114 AND `check_status` = 1 AND `category_id` = 147 ORDER BY `submit_date` ASC LIMIT 1 [ RunTime:0.002497s ]
[ SQL ] SELECT `id`,`title` FROM `tb_tech` WHERE `id` < 855114 AND `check_status` = 1 AND `category_id` = 147 ORDER BY `submit_date` DESC LIMIT 1 [ RunTime:0.131616s ]
[ SQL ] select id,title,tag,submit_date from tb_tech where check_status=1 and id<>855114 order by submit_date desc limit 10 [ RunTime:0.003916s ]
[ SQL ] SHOW COLUMNS FROM `tb_mcc` [ RunTime:0.014930s ]
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[ SQL ] SHOW COLUMNS FROM `tb_industry_report` [ RunTime:0.001020s ]
[ SQL ] SELECT `id`,`cate_aid`,`cate_bid`,`title`,`pic`,`tag`,`price`,`hit`,`mode` FROM `tb_industry_report` [ RunTime:0.001111s ]
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[ SQL ] SELECT * FROM `tb_advert` WHERE `id` = 65 LIMIT 1 [ RunTime:0.000853s ]
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