本发明公开了一种化学合成的HBV 1.2x基因组、表达系统及构建方法，所述HBV 1.2x基因组的DNA序列为SEQ ID NO：1所示的第一序列。所述第一序列由SEQ ID NO：2所示的第二序列改造而成，所述改造序列(对应第二序列的1501-3215，1-2150)，其中1501-3215片段的第1909个核苷酸由T突变为C、第1912个核苷酸由C突变为T、第2248个核苷酸由T突变为C，在改造序列的两端均加入序列GATATC得到所述第一序列。根据本发明HBV1.2x基因组构建重组质粒pcDNA3.1(+)/HBV，然后转入肝肿瘤细胞系HepG2细胞，培养上清中检测到HBV特异性抗原HBe Ag和HBs Ag，从而能够实现HBV特异性抗原的表达。
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[8]Array to string conversion[/websites/wwwroot/www.china-mcc.com/thinkphp/library/think/Url.php:147]
[8]Array to string conversion[/websites/wwwroot/www.china-mcc.com/thinkphp/library/think/Url.php:147]
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[8]Array to string conversion[/websites/wwwroot/www.china-mcc.com/thinkphp/library/think/Url.php:147]
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[ DB ] CONNECT:[ UseTime:0.001444s ] mysql:host=pc-m5epcw1px949b110u.rwlb.rds.aliyuncs.com;port=3306;dbname=yousedb;charset=utf8
[ SQL ] SHOW COLUMNS FROM `tb_para` [ RunTime:0.002123s ]
[ SQL ] SELECT * FROM `tb_para` WHERE `id` = 1 LIMIT 1 [ RunTime:0.001329s ]
[ SQL ] SHOW COLUMNS FROM `tb_tech` [ RunTime:0.001310s ]
[ SQL ] UPDATE `tb_tech` SET `hit`=`hit`+1 WHERE `id` = 855114 [ RunTime:0.001268s ]
[ SQL ] SELECT * FROM `tb_tech` WHERE `id` = 855114 LIMIT 1 [ RunTime:0.001099s ]
[ SQL ] SHOW COLUMNS FROM `tb_admin` [ RunTime:0.001112s ]
[ SQL ] SELECT `realname` FROM `tb_admin` WHERE `id` = 1 LIMIT 1 [ RunTime:0.000838s ]
[ SQL ] SHOW COLUMNS FROM `tb_category` [ RunTime:0.001171s ]
[ SQL ] SELECT * FROM `tb_category` WHERE `id` = 147 LIMIT 1 [ RunTime:0.000844s ]
[ SQL ] SELECT * FROM `tb_category` WHERE `id` = 30 LIMIT 1 [ RunTime:0.000861s ]
[ SQL ] SHOW COLUMNS FROM `tb_anchor` [ RunTime:0.001099s ]
[ SQL ] SELECT * FROM `tb_anchor` ORDER BY `id` ASC [ RunTime:0.001600s ]
[ SQL ] SELECT `id`,`title` FROM `tb_tech` WHERE `id` > 855114 AND `check_status` = 1 AND `category_id` = 147 ORDER BY `submit_date` ASC LIMIT 1 [ RunTime:0.002481s ]
[ SQL ] SELECT `id`,`title` FROM `tb_tech` WHERE `id` < 855114 AND `check_status` = 1 AND `category_id` = 147 ORDER BY `submit_date` DESC LIMIT 1 [ RunTime:0.149262s ]
[ SQL ] SHOW COLUMNS FROM `tb_mcc` [ RunTime:0.001129s ]
[ SQL ] SELECT * FROM `tb_mcc` WHERE `classify` = 1 [ RunTime:0.000877s ]
[ SQL ] SELECT * FROM `tb_mcc` WHERE `classify` = 2 [ RunTime:0.000860s ]
[ DB ] CONNECT:[ UseTime:0.001273s ] mysql:host=mccbfzy.mysql.polardb.rds.aliyuncs.com;port=3306;dbname=ysscdb;charset=utf8
[ SQL ] SHOW COLUMNS FROM `tb_industry_report` [ RunTime:0.001064s ]
[ SQL ] SELECT `id`,`cate_aid`,`cate_bid`,`title`,`pic`,`tag`,`price`,`hit`,`mode` FROM `tb_industry_report` [ RunTime:0.001118s ]
[ SQL ] SELECT * FROM `tb_category` WHERE `pid` = 36 ORDER BY `sortid` ASC [ RunTime:0.000982s ]
[ SQL ] SELECT * FROM `tb_category` WHERE `pid` = 37 ORDER BY `sortid` ASC [ RunTime:0.000921s ]
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[ SQL ] SELECT * FROM `tb_category` WHERE `pid` = 40 ORDER BY `sortid` ASC [ RunTime:0.001636s ]
[ SQL ] SELECT * FROM `tb_category` WHERE `pid` = 41 ORDER BY `sortid` ASC [ RunTime:0.000850s ]
[ SQL ] SHOW COLUMNS FROM `tb_advert` [ RunTime:0.001131s ]
[ SQL ] SELECT * FROM `tb_advert` WHERE `id` = 62 LIMIT 1 [ RunTime:0.000847s ]
[ SQL ] SELECT * FROM `tb_advert` WHERE `id` = 63 LIMIT 1 [ RunTime:0.000885s ]
[ SQL ] SELECT * FROM `tb_advert` WHERE `id` = 64 LIMIT 1 [ RunTime:0.000840s ]
[ SQL ] SELECT * FROM `tb_advert` WHERE `id` = 65 LIMIT 1 [ RunTime:0.000865s ]
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