本发明公开了一种基于高响应葡萄糖适配体的电化学传感器，所述检测传感器包括电极，所述电极外附有葡萄糖检测核酸适配体和巯基己醇单分子层；所述葡萄糖检测核酸适配体包括能与葡萄糖进行特异性结合的人工合成核苷酸序列，以及修饰基团和亚甲基蓝基团，所述修饰基团为HO‑C6‑S‑S‑C6‑；所述能与葡萄糖进行特异性结合的人工合成核苷酸序列为序列SEQ ID NO.1、SEQ ID NO.2、SEQ ID NO.3或SEQ ID NO.4中的一种。本发明还保护上述传感器的制备方法。本发明采用核酸适配体作为识别基元,能在全血环境中高灵敏度地检测葡萄糖，不需要任何酶的参与，检测过程十分便捷。
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