本发明提供了一种检测猪伪狂犬病毒野毒抗体的试剂盒及检测方法。所述试剂盒包括以猪伪狂犬病病毒HB1201毒株为模板，利用上下游引物进行PCR扩增得到的重组蛋白；所述上游引物的核苷酸序列如SEQ ID NO.1所示；所述下游引物的核苷酸序列如SEQ ID NO.2所示。本发明提供的含有gE重组蛋白的猪伪狂犬病毒野毒抗体的试剂盒，与商品化ELISA试剂盒相比，具有更高的检测敏感性。本发明建立的PRV gE抗体检测间接化学发光酶免疫方法为我国猪群中猪伪狂犬病野毒感染情况调查提供了敏感的检测手段，可用于病毒感染的早期、快速诊断，具有较好的开发前景。
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