本发明涉及生物技术领域，为解决目前蛋白的快速实时检测中ELISA等检测方法复杂耗时费试剂的缺点、电化学检测方法的非特异性吸附和界面修饰等缺点，本发明提出了一种蛋白定量检测方法，将待测蛋白样本、调节探针、扩增模板、切口酶、DNA聚合酶、缓冲液、dNTPs、氯化镁、SYBR Green I荧光染料制成混合溶液，在等温条件下进行扩增反应，将待测蛋白浓度与等温扩增曲线的出峰时间之间建立定量关系，实现蛋白的实时定量检测。本方法具有简单、特异性高、通用性强和可以实时定量检测等优势，其检测限能达到亚纳摩尔级，可以满足一般的蛋白检测要求。
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[ SQL ] SELECT `id`,`title` FROM `tb_tech` WHERE `id` < 866367 AND `check_status` = 1 AND `category_id` = 147 ORDER BY `submit_date` DESC LIMIT 1 [ RunTime:0.095001s ]
[ SQL ] select id,title,tag,submit_date from tb_tech where check_status=1 and id<>866367 order by submit_date desc limit 10 [ RunTime:0.004384s ]
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