本发明公开了一株抗HPV16 E6蛋白的单克隆抗体。本发明的单克隆抗体的特征为具有氨基酸序列如SEQ ID NO：4、SEQ ID NO：6和SEQ ID NO：8所示的重链可变区、具有氨基酸序列如SEQ ID NO：10、SEQ ID NO：12和SEQ ID NO：14所示的轻链可变区。免疫印记实验显示该抗体能特异识别重组的HPV16 E6蛋白以及表达HPV16 E6蛋白的宫颈癌组织。该抗体可以用于通过人工或自动化方式，以免疫组织化学染色(IHC)、酶联免疫吸附(ELISA)或免疫印迹(Western Blot)方式检测细胞中HPV16 E6蛋白的表达水平，可特异性诊断HPV16病毒，这为在蛋白水平上检测HPV16型病毒提供了新手段，并可用于人类生殖系统传染性疾病及其肿瘤的研究，亦可用于食管癌、乳腺癌、肛门癌等相关恶性肿瘤的研究。
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[ DB ] CONNECT:[ UseTime:0.001225s ] mysql:host=pc-m5epcw1px949b110u.rwlb.rds.aliyuncs.com;port=3306;dbname=yousedb;charset=utf8
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[ SQL ] SELECT * FROM `tb_para` WHERE `id` = 1 LIMIT 1 [ RunTime:0.001078s ]
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[ SQL ] UPDATE `tb_tech` SET `hit`=`hit`+1 WHERE `id` = 883333 [ RunTime:0.001118s ]
[ SQL ] SELECT * FROM `tb_tech` WHERE `id` = 883333 LIMIT 1 [ RunTime:0.000804s ]
[ SQL ] SHOW COLUMNS FROM `tb_admin` [ RunTime:0.001017s ]
[ SQL ] SELECT `realname` FROM `tb_admin` WHERE `id` = 1 LIMIT 1 [ RunTime:0.000714s ]
[ SQL ] SHOW COLUMNS FROM `tb_category` [ RunTime:0.001126s ]
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[ SQL ] SELECT * FROM `tb_category` WHERE `id` = 30 LIMIT 1 [ RunTime:0.000696s ]
[ SQL ] SHOW COLUMNS FROM `tb_anchor` [ RunTime:0.000957s ]
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[ SQL ] SELECT `id`,`title` FROM `tb_tech` WHERE `id` > 883333 AND `check_status` = 1 AND `category_id` = 147 ORDER BY `submit_date` ASC LIMIT 1 [ RunTime:0.007185s ]
[ SQL ] SELECT `id`,`title` FROM `tb_tech` WHERE `id` < 883333 AND `check_status` = 1 AND `category_id` = 147 ORDER BY `submit_date` DESC LIMIT 1 [ RunTime:0.095350s ]
[ SQL ] select id,title,tag,submit_date from tb_tech where check_status=1 and id<>883333 order by submit_date desc limit 10 [ RunTime:0.004375s ]
[ SQL ] SHOW COLUMNS FROM `tb_mcc` [ RunTime:0.001241s ]
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[ SQL ] SHOW COLUMNS FROM `tb_industry_report` [ RunTime:0.001076s ]
[ SQL ] SELECT `id`,`cate_aid`,`cate_bid`,`title`,`pic`,`tag`,`price`,`hit`,`mode` FROM `tb_industry_report` [ RunTime:0.001519s ]
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[ SQL ] SELECT * FROM `tb_category` WHERE `pid` = 41 ORDER BY `sortid` ASC [ RunTime:0.000988s ]
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[ SQL ] SELECT * FROM `tb_advert` WHERE `id` = 65 LIMIT 1 [ RunTime:0.000737s ]
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