本发明公开了一种用于识别且增强HMGB1生物学活性的核酸适配子及其应用，通过SELEX方法成功筛选了特异性识别蛋白质HMGB1的核酸适配子。结果表明，所筛选的核酸适配子可以特异性识别并结合蛋白质HMGB1，且具有较高的特异性及灵敏性。经过生物素标记后的核酸适配子可以应用于核酸蛋白质印迹(South‑Western blotting)、基于适配子的酶联吸附试验(ELASA)、细胞及组织化学染色(Aptamer‑based histochemistry and cytochemistry)。核酸适配子在亲和力和特异性方面可与抗体媲美，甚至优于抗体，有望被开发成为体内外检测蛋白质HMGB1的有力工具。
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[ DB ] CONNECT:[ UseTime:0.001281s ] mysql:host=pc-m5epcw1px949b110u.rwlb.rds.aliyuncs.com;port=3306;dbname=yousedb;charset=utf8
[ SQL ] SHOW COLUMNS FROM `tb_para` [ RunTime:0.001709s ]
[ SQL ] SELECT * FROM `tb_para` WHERE `id` = 1 LIMIT 1 [ RunTime:0.001719s ]
[ SQL ] SHOW COLUMNS FROM `tb_tech` [ RunTime:0.001224s ]
[ SQL ] UPDATE `tb_tech` SET `hit`=`hit`+1 WHERE `id` = 893685 [ RunTime:0.001201s ]
[ SQL ] SELECT * FROM `tb_tech` WHERE `id` = 893685 LIMIT 1 [ RunTime:0.001044s ]
[ SQL ] SHOW COLUMNS FROM `tb_admin` [ RunTime:0.001136s ]
[ SQL ] SELECT `realname` FROM `tb_admin` WHERE `id` = 1 LIMIT 1 [ RunTime:0.000902s ]
[ SQL ] SHOW COLUMNS FROM `tb_category` [ RunTime:0.001237s ]
[ SQL ] SELECT * FROM `tb_category` WHERE `id` = 147 LIMIT 1 [ RunTime:0.000981s ]
[ SQL ] SELECT * FROM `tb_category` WHERE `id` = 30 LIMIT 1 [ RunTime:0.001224s ]
[ SQL ] SHOW COLUMNS FROM `tb_anchor` [ RunTime:0.001429s ]
[ SQL ] SELECT * FROM `tb_anchor` ORDER BY `id` ASC [ RunTime:0.003299s ]
[ SQL ] SELECT `id`,`title` FROM `tb_tech` WHERE `id` > 893685 AND `check_status` = 1 AND `category_id` = 147 ORDER BY `submit_date` ASC LIMIT 1 [ RunTime:0.017357s ]
[ SQL ] SELECT `id`,`title` FROM `tb_tech` WHERE `id` < 893685 AND `check_status` = 1 AND `category_id` = 147 ORDER BY `submit_date` DESC LIMIT 1 [ RunTime:0.024068s ]
[ SQL ] select id,title,tag,submit_date from tb_tech where check_status=1 and id<>893685 order by submit_date desc limit 10 [ RunTime:0.006460s ]
[ SQL ] SHOW COLUMNS FROM `tb_mcc` [ RunTime:0.000961s ]
[ SQL ] SELECT * FROM `tb_mcc` WHERE `classify` = 1 [ RunTime:0.000797s ]
[ SQL ] SELECT * FROM `tb_mcc` WHERE `classify` = 2 [ RunTime:0.001868s ]
[ DB ] CONNECT:[ UseTime:0.001996s ] mysql:host=mccbfzy.mysql.polardb.rds.aliyuncs.com;port=3306;dbname=ysscdb;charset=utf8
[ SQL ] SHOW COLUMNS FROM `tb_industry_report` [ RunTime:0.001330s ]
[ SQL ] SELECT `id`,`cate_aid`,`cate_bid`,`title`,`pic`,`tag`,`price`,`hit`,`mode` FROM `tb_industry_report` [ RunTime:0.001597s ]
[ SQL ] SELECT * FROM `tb_category` WHERE `pid` = 36 ORDER BY `sortid` ASC [ RunTime:0.000848s ]
[ SQL ] SELECT * FROM `tb_category` WHERE `pid` = 37 ORDER BY `sortid` ASC [ RunTime:0.001928s ]
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[ SQL ] SELECT * FROM `tb_category` WHERE `pid` = 40 ORDER BY `sortid` ASC [ RunTime:0.000879s ]
[ SQL ] SELECT * FROM `tb_category` WHERE `pid` = 41 ORDER BY `sortid` ASC [ RunTime:0.001488s ]
[ SQL ] SHOW COLUMNS FROM `tb_advert` [ RunTime:0.010004s ]
[ SQL ] SELECT * FROM `tb_advert` WHERE `id` = 62 LIMIT 1 [ RunTime:0.000856s ]
[ SQL ] SELECT * FROM `tb_advert` WHERE `id` = 63 LIMIT 1 [ RunTime:0.000938s ]
[ SQL ] SELECT * FROM `tb_advert` WHERE `id` = 64 LIMIT 1 [ RunTime:0.003802s ]
[ SQL ] SELECT * FROM `tb_advert` WHERE `id` = 65 LIMIT 1 [ RunTime:0.000771s ]
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