本发明提供了一种猪小肠上皮细胞表达的PGC启动子及其应用，该PGC启动子核苷酸序列如SEQ ID No.1所示，本发明通过在线网站预测PGC可靠启动子区；化学合成方法合成引物；通过限制性酶切位点MluⅠ和NheⅠ，将PGC启动子序列插入pGL3‑Basic载体，得到PGC‑双荧光素酶重组质粒；转染大肠杆菌进行质粒扩增，提取质粒，测序验证；转染入猪小肠上皮细胞，发现猪源PGC启动子可在猪小肠上皮细胞中启动双荧光素酶基因表达，说明所述启动子能够调控下游基因在猪小肠上皮细胞中表达，有利于构建稳定高效的小肠转录调控元件，在制备转基因动物和小肠疾病基因治疗研究中具有应用价值。
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[ DB ] CONNECT:[ UseTime:0.001303s ] mysql:host=pc-m5epcw1px949b110u.rwlb.rds.aliyuncs.com;port=3306;dbname=yousedb;charset=utf8
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[ SQL ] SELECT * FROM `tb_anchor` ORDER BY `id` ASC [ RunTime:0.001768s ]
[ SQL ] SELECT `id`,`title` FROM `tb_tech` WHERE `id` > 897131 AND `check_status` = 1 AND `category_id` = 147 ORDER BY `submit_date` ASC LIMIT 1 [ RunTime:0.008090s ]
[ SQL ] SELECT `id`,`title` FROM `tb_tech` WHERE `id` < 897131 AND `check_status` = 1 AND `category_id` = 147 ORDER BY `submit_date` DESC LIMIT 1 [ RunTime:0.013599s ]
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[ SQL ] SHOW COLUMNS FROM `tb_industry_report` [ RunTime:0.001174s ]
[ SQL ] SELECT `id`,`cate_aid`,`cate_bid`,`title`,`pic`,`tag`,`price`,`hit`,`mode` FROM `tb_industry_report` [ RunTime:0.001238s ]
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