本发明属于人工金属酶领域，公开了一种高效的脱色过氧化物酶及其制备方法与应用。本发明所述脱色过氧化物酶是利用运输氧功能的肌红蛋白作为蛋白质分子设计骨架，在肌红蛋白血红素活性中心附近43位引入酪氨酸、138位引入色氨酸、且同时在血红素活性中心外围88位引入色氨酸获得三点突变体蛋白，其氨基酸序列如SEQ ID NO.1所示。本发明所述脱色过氧化物酶提高了催化转化数，降低了对过氧化氢的依耐性，其制备方法操作简单，适合大规模产业化生产。实验表明，本发明所述脱色过氧化物酶在低过氧化氢浓度下数秒内可降解染料分子，达到脱色效果，催化效率比双点突变体蛋白增加了130％，可以广泛应用到工业染料废水的污染治理中。
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