本发明公开了一种三元穿梭载体pTY，其序列如SEQ ID NO:11所示。还公开了一种利用pTY构建柑桔黄化脉明病毒(CYVCV)侵染性克隆的方法，包括如下步骤：(1)制备CYVCV全长cDNA；(2)采用限制性内切酶Stu I、Sma I酶切如SEQ ID NO:11所示三元穿梭载体pTY，得到pTY线性化的载体；(3)酵母转化伴随的同源重组：采用醋酸锂法将线性化载体pTY与CYVCV全长cDNA共转化酵母菌YPH501；(4)侵染性克隆鉴定：同源重组质粒转化农杆菌C58C1，筛选获得CYVCV侵染性克隆。本发明有效克服了病毒全长cDNA片段在大肠杆菌中产生毒性或不稳定导致克隆失败的现象。
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[ DB ] CONNECT:[ UseTime:0.001293s ] mysql:host=pc-m5epcw1px949b110u.rwlb.rds.aliyuncs.com;port=3306;dbname=yousedb;charset=utf8
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[ SQL ] SHOW COLUMNS FROM `tb_industry_report` [ RunTime:0.001013s ]
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