本发明涉及一种可重复使用的以纳米金刚石为基质的磷酸化肽吸附材料及其制备和应用。具体是以表面带有氨基的纳米金刚石为基质，3,4,5‑三羟基苯甲醛为修饰剂，采用氨醛缩合反应在金刚石微粒表面引入联苯三酚基团，该基团作为配体可以与锆离子(Zr4+)进行螯合，所得到的材料可以用作固定化金属离子亲和色谱(Immobilized metal affinity chromatography,IMAC)的吸附剂，用来富集复杂生物样品中的磷酸肽。由于Zr4+与联苯三酚之间的螯合作用力大于其与磷酸基团之间的螯合作用力，在富集完成之后，通过使用恰当的碱性洗脱剂，可使磷酸肽从吸附剂上洗脱的同时保留吸附剂上螯合的Zr4+，因此，用酸性溶液重新平衡后，吸附剂可再次进行使用。
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